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The determination of early apoptosis was done at 24 h by aspirating the MM cells away from the BMSC and using Annexin V assay with flow cytometry and cytotoxicity at 96 h using DIMSCAN assay as previously described

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Thus, 5OP is a signal to which Salmonella directly responds to

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With the proper documentation and approvals, they can then be cleared to assist with or independently perform IV insertions

These approaches align with the living lab concept, emphasising co-creation, experimentation, and real-world testing in collaboration with stakeholders